Talk to yo later
Peter
On Wed, 13 Nov 1996, Simon Barry wrote:
> Peter,
>
> > This is still not a brilliant solution as the hardness changes
> > continuosly. What we really need is for someone on this list who has had
> > success with the shel grit method to set up two tanks with eggs split
> > from the one spawning, add CaCl2 to one and NaHCO3 to the other. Then we
> > may finally be able to work out what Roy and Gary have been argueing
> > about.
>
> This design is not the best. The effect of the different tanks is totally confounded with the water treatment. It thus does not provide a useful comparison as we cannot determine if any observed differences are due to the differences in tank position/
temperature/size etc or the water treatment.
>
> A better experiment is to replicate the tanks, for example treat two tanks with CaCl2 and two with NaHCO3. We can then compare any differences observed with the tank to tank variation. This will allow us much greater confidence in assigning the water
treatment to any observed differences in rates of deformity.
>
>
>
> > if you wanted it could also be extended to include four tanks, one
> > plus and minus trace elements for each treatment.
> >
>
> See above.
>
> Cheers
>
> Simon Barry
>